The estimation of antipyrine in biological materials.

نویسندگان

  • B B BRODIE
  • J AXELROD
چکیده

A means of measuring the total water of the body in vivo has been developed (1). This method involves the use of antipyrine (l-phenyl-2,3dimethylpyrazolone-5-one), the well known analgesic and antipyretic. Two methods for the determination of this compound in biological materials are described in this report. The first, an extraction procedure, is suitable for its estimation in biological fluids and tissues. In this procedure antipyrine is extracted from the biological material with chloroform, the solvent evaporated, and the residue dissolved in dilute sulfuric acid. Sodium nitrite is added and the resulting 4-nitrosoantipyrine measured in a spectrophotometer at 350 mp. This procedure, as indicated by a distribution technique (2, 3), possesses a high degree of specificity. The second method, suitable for plasma, involves the estimation of the antipyrine directly in the plasma filtrate after deproteinization with zinc hydroxide. The speed and simplicity of this method recommend it for routine use. This procedure also has a high degree of specificity.

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عنوان ژورنال:
  • The Journal of biological chemistry

دوره 179 1  شماره 

صفحات  -

تاریخ انتشار 1949